期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1292, 期 -, 页码 201-210出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.01.011
关键词
Alkylamine; Ion-pairing reagent; Oligosaccharide characterization; Isomer resolution; Heparin quality assurance
资金
- National Science Foundation [CHE-0848976, -1213845]
- National Institutes of Health [CA138535]
- Direct For Mathematical & Physical Scien [1213845] Funding Source: National Science Foundation
- Division Of Chemistry [1213845] Funding Source: National Science Foundation
Heparin is a complex mixture of sulfated linear carbohydrate polymers. It is widely used as an antithrombotic drug, though it has been shown to have a myriad of additional biological activities. Heparin is often partially depolymerized in order to decrease the average molecular weight, as it has been shown that low molecular weight heparins (LMWH) possess more desirable pharmacokinetic and pharmacodynamic properties than unfractionated heparin (UFH). Due to the prevalence of LMWHs in the market and the emerging availability of generic LMWH products, it is important that analytical methods be developed to ensure the drug quality. This work explores the use of tributylamine (TrBA), dibutylamine (DBA), and pentylamine (PTA) as ion-pairing reagents in conjunction with acetonitrile and methanol modified mobile phases for reversed-phase ion-pairing ultraperformance liquid chromatography coupled to mass spectrometry (RPIP-UPLC MS) for fingerprint analysis of LMWH preparations. RPIP-UPLC MS fingerprints are presented and compared for tinzaparinand enoxaparin. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据