期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1306, 期 -, 页码 27-36出版社
ELSEVIER
DOI: 10.1016/j.chroma.2013.07.048
关键词
Comprehensive two-dimensional liquid chromatography; Peptides; RPLC x RPLC; RPLC x HILIC; Protein tryptic digest
In this second paper of a two-part series, on-line RPLC x HILIC is compared to on-line RPLC x RPLC through the separation of peptides. Our choices regarding the conditions are discussed. Injection effects and overloading effects are evaluated in both configurations. It is shown that whereas large volumes can be injected in the second dimension in RPLC x RPLC under HT-UHPLC conditions (>20% of the column dead volume), even small injection volumes (8% of the column the dead volume) have a detrimental effect on peak shapes in RPLC x HILIC. Advantages and limits of the two 2D-systems are compared through the 2D-separation of a tryptic digest of three proteins. A ten-fold gain in analysis time along with a significant gain in peak capacity are obtained with both systems compared to the most efficient one-dimensional separation of peptides recently published. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据