期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1311, 期 -, 页码 30-40出版社
ELSEVIER
DOI: 10.1016/j.chroma.2013.08.078
关键词
Flavonoid; Citrus fruit juice; Human plasma; Urine sample; Miniaturized ultrasound-assisted MSPD; HPLC
资金
- Semnan University Research Council
- institute of standard and industrial research of Iran (Khorasan-e-Razavi province)
In the present study, for the first time, a simplified miniaturized ultrasound-assisted matrix solid-phase dispersion (SM-USA-MSPD) method with a different application for liquid matrices was developed to extract different flavonoids (hesperidin, diosmin, eriocitrin, narirutin, naringin, hesperetin and naringenin) from citrus fruit juice and human fluid samples prior to their determination using high performance liquid chromatography (HPLC). Different effective parameters were studied and under the optimum conditions (including sample volume: 150 mu L; solid phase: silica-based C18, 200 mg; eluting solvent: methanol, 500 mu L.; pH: 4; and sonication: 6 min; at room temperature), limits of detection and limits of quantification were ranged from 23.3 to 46.8 ng mL(-1) and 74.8 to 141.5 ng mL(-1), respectively. Once optimized, analytical performance of the method was studied in terms of linearity (0.074-198.5 mu g mL(-1), r(2) >0.991), accuracy (recovery=84.6-101.5%), and precision (repeatability: intra-day precision <5.9%, and inter-day precision <7.2%). At the end, SM-USA-MSPD method was successfully applied to estimate the levels of hesperetin and naringenin in plasma and urinary excretion -after ingestion of orange, grapefruit and lime juices- and the obtained results confirmed that these compounds could be used as good biomarkers of citrus fruit juice intake. (C) 2013 Elsevier B.V. All rights reserved.
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