期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1310, 期 -, 页码 82-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.08.069
关键词
Two-dimensional chromatography; SAX/RPLC; 2D LC-MS; Lipidomics; Tandem mass spectrometry
资金
- National Research Foundation of Korea [2012-0005598]
- Korean government
An on-line two-dimensional liquid chromatography method was developed for comprehensive lipid profiling by coupling strong anion exchange (SAX) and nanoflow reversed-phase liquid chromatography (nRPLC) prior to electrospray ionization-tandem mass spectrometry (2D-SAX/nRPLC-ESI-MS/MS). Lipids can be classified into four different types according to the electrical propensities of the lipids: anionic, weak anionic, neutral polar, and special lipids. In 2D-SAX/nRPLC, various lipids can be fractionated in the first dimension (SAX: 5 mu m to 100 angstrom, 5.0 cm x 75 mu m i.d.) by step elution (methanol and salt solution), followed by the molecular separation of lipids in the second dimension (RP: 3 mu m to 100 angstrom, 7.0 cm x 75 mu m i.d.) with binary gradient LC. Since the elution of lipids from SAX can be achieved with a very small volume of eluent delivered from an autosampler, it can be simply implemented with an LC-ESI-MS instrument for full automation, and the salt step elution, including the two-step injection procedure, can be used for the selective analysis of the desired lipid fraction. For nRPLC-ESI-MS/MS run in either positive or negative ion mode, a common ionization modifier (0.05% ammonium hydroxide with 5 mM ammonium formate) was introduced into the binary mobile phase solutions so that 2D-LC-MS could be operated in both ion modes without changing the mobile phase solutions. The developed on-line 2D-SAX/nRPLC-ESI-MS/MS was evaluated with 22 different standard lipids for the optimization of the salt step elution and was applied to a healthy human plasma lipid extract, resulting in the identification of a total of 303 plasma lipids, including 14 different classes. (C) 2013 Elsevier B.V. All rights reserved.
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