期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1282, 期 -, 页码 1-10出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.01.041
关键词
Biogenic amine; In situ derivatization; Benzoylation; Dispersive liquid-liquid microextraction; Solidification of floating organic droplets; Alcoholic beverage
资金
- SEOK CHUN Research Fund, Sungkyunkwan University
- Basic Science Research Program of the National Research Foundation of Korea (NRF) [2011-0024225]
- Ministry of Education, Science, and Technology (MEST), Republic of Korea
- National Research Foundation of Korea [2011-0024225] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A novel analytical method consisting of in situ derivatization combined with liquid phase microextraction followed by liquid chromatography-ultraviolet detection (LC-UV) was developed to determine the biogenic amines (BAs) of alcoholic beverages. Nine BAs (putrescine, cadaverine, 1,3-diaminopropane, tryptamine, phenylethylamine, spermidine, spermine, histamine, and tyramine) were derivatized in situ with benzoyl chloride, extracted by dispersive liquid-liquid microextraction based on solidification of floating organic droplets (DLLME-SFO), and then chromatographed by LC-UV. Factors influencing the derivatization and extraction efficiency were optimized, including the reaction buffer pH and concentration, amount of derivatization reagent, reaction time, types and volumes of extraction and dispersive solvents, and extraction time. Under the optimized conditions, the method was linear over 0.05-8.0 mu g mL(-1) with an r(2) >= 0.992 and exhibited intra- and inter-day precision less than 8.8% and 11.5%, respectively. The limit of detection ranged between 0.005 and 0.011.mu g mL(-1). The developed method using a basic LC-UV system is sensitive, rapid, convenient, green, and cost-effective. Moreover, it is versatile and practical for the analysis of BAs, as demonstrated by the successful application in four different types of popular alcoholic beverages (white wine, red wine, rice wine, and beer). (C) 2013 Elsevier B.V. All rights reserved.
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