期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1230, 期 -, 页码 54-60出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2012.01.065
关键词
Capillary liquid chromatography; Organic polymer monolith; Hydrophilic liquid chromatography; Poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolith
资金
- National Science Fund for Distinguished Young Scholars [20625516]
- National Natural Science Foundation [91017013, 31070327]
- NSFC [20921062]
In current study, poly(methacrylic acid-co-ethylene glycol dimethacrylate) (MAA-co-EDMA) monolith was successfully prepared for capillary hydrophilic liquid chromatography (cHILIC). The polymerization was optimized by changing the ratio of MAA to EDMA, the type and amount of porogen. The characterization indicated that hydrophilic monolithic column possessed homogeneous column bed, good permeability and narrow pore size distribution. Under HILIC mode, the hydrophilic monolith prepared with PEG and DMSO showed stronger hydrophilicity than the hydrophobic monolith prepared with dodecanol and toluene. Finally, the hydrophilic monolith was applied in the separation of tryptic digests of bovine serum albumin (BSA) with cHILIC-ESI-qTOF-MS system. Our results revealed that 49 peptides were identified with 50% sequence coverage under HILIC mode, which was much better than the peptides identified using particulate-packed commercial column with RPLC-ESI-qTOF-MS system or hydrophobic monolith with cHILIC-ESI-qTOF-MS system. Taken together, the hydrophilic monolithic column prepared in current study, demonstrated the excellent chromatographic performance on the separation of complex samples, which offers the potential application of the monolith on proteomics study. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据