期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1267, 期 -, 页码 109-115出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2012.06.053
关键词
Complex-formation equilibria; Enantiomer separations; PeakMaster 5.3; Electrophoresis; Nonlinear electromigration
资金
- Grant Agency of the Czech Republic [203/08/1428]
- Grant Agency of Charles University [323611, 669412]
- Ministry of Education of the Czech Republic [MSM 0021620857]
The complete mathematical model of electromigration dispersion in systems that contain a neutral complex forming agent and a fully charged analyte was introduced in the previous part of this series of papers (Part III - Theory). The model was implemented in the newest version of our simulation program PeakMaster 5.3 that calculates the effective mobility of the analyte and its nonlinear electromigration mobility slope, S-EMD, in the presence of a complex forming agent in the background electrolyte. The mathematical model was verified by both experiments and simulations, which were performed by our dynamic simulator Simul 5 Complex. Three separation systems differing in the chiral selector used (having different values for the complexation constant and the mobility of the complex) were chosen for the verification. The nonlinear electromigration mobility slope values were calculated from the simulations and the experiments that were performed at different complex forming agent concentrations. These data agree very well with those predicted by the mathematical model and provided the foundation for the discussion and explanation of the electromigration dispersion process that occurs in systems which contain a complex forming agent. The new version of PeakMaster 5.3 was shown to be a powerful tool for optimization of the separation conditions by minimizing electromigration dispersion which improves the symmetry of the analyte peaks and their resolution. (C) 2012 Elsevier B.V. All rights reserved.
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