期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1251, 期 -, 页码 82-90出版社
ELSEVIER
DOI: 10.1016/j.chroma.2012.06.032
关键词
Capillary liquid chromatography; Mixed-mode polymer monolith; 4-Vinylphenylboronic acid; Small molecules; Proteins
资金
- National Basic Research Program of China [2010CB732403]
- NSFC [21005018, 21075016]
- Doctoral Fund of Ministry of Education of China [20103514120002]
- Natural Science Foundation of Fujian Province, China [2010J01039]
- Foundation of Fujian Educational Committee [JA10030]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
- Technology Development Fund of Fuzhou University [2010-XQ-07]
- Talent Startup Fund of Fuzhou University [0460022233]
A mixed-mode monolithic stationary phase was prepared for capillary liquid chromatography (cLC) by in situ copolymerization of 4-vinylphenylboronic acid (VPBA) and pentaerythritol triacrylate (PETA) in a binary porogenic solvent consisting of ethylene glycol/cyclohexanol. The monomer of VPBA functioned as ion-exchange sites, hydrophilic ligands, hydrophobic groups and affinity sites, while PETA was introduced as a hydrophilic crosslinker. The resultant monoliths with different column properties (e.g. morphology, permeability and selectivity) were optimized by adjusting the ratio of VPBA to PETA and the composition of porogenic solvent. The results showed that the selectivity of the monoliths increased with increasing content of VPBA in the polymerization mixture. A series of alkylbenzenes, amides, and anilines were used to evaluate the column performance in terms of hydrophobic, hydrophilic and cation-exchange interactions. At an optimized flow rate of 50 mu L/min (corresponding to 0.265 mm/s), the monolith exhibited high column efficiencies of 43,000-100,000 plates/m for alkylbenzenes. Good repeatability was obtained with relative standard deviation (RSD) of retention factor (k) less than 0.65% for run-to-run (n = 5) and less than 2.49% for column-to-column (n = 5). In addition, the poly(VPBA-co-PETA) monolithic column was applied to the separation of phenols, nucleobases, and proteins, respectively. These successful applications demonstrate the purposed monoliths are promising for cLC separation of small molecules and proteins. (c) 2012 Elsevier B.V. All rights reserved.
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