期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1256, 期 -, 页码 1-8出版社
ELSEVIER
DOI: 10.1016/j.chroma.2012.07.037
关键词
Matrix solid-phase dispersion; Dummy imprinted polymers; Auxin; High performance liquid chromatography; Orange samples
资金
- National Natural Science Foundation of China [20905019, 21011140338]
- Natural Science Foundation of Hebei [B2010000209]
- Science Foundation of Education Department of Hebei Province [CPRC003]
A simple, convenient and high selective miniaturized molecularly imprinted matrix solid-phase dispersion (mini-MI-MSPD) technique using L-tryptophan-imprinted polymers as sorbents was proposed for simultaneous extraction of four auxins (indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)) from orange samples coupled with high performance liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized using L-tryptophan as dummy template showed high affinity and selectivity to auxins, and so they were applied as the specific dispersant of MSPD to simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.02-2.00 mu g g(-1) and the average recoveries of four auxins at three spiked levels ranged from 87.6% to 104.5% with the relative standard deviation (RSD) <= 4.4%. The presented mini-MI-MSPD method combined the advantages of MSPD for allowing the extraction, dispersion and homogenization in a single step and the advantages of MIPs for high affinity and selectivity towards four auxins, which could be applied to the determination of auxins in complicated vegetal samples. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据