期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1266, 期 -, 页码 43-52出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2012.10.004
关键词
Peptides; Peptide-silica conjugate; RP-H PLC; HILIC
资金
- Grants-in-Aid for Scientific Research [23245018] Funding Source: KAKEN
The short peptide Boc-Phe-Aib-Phe-OH was synthesized and immobilized onto porous silica using grafting methodology. The resulting peptide-bonded silica was characterized using DRIFT-mode FT-IR, elemental analysis, thermogravimetric analysis, solid state C-13 NMR spectroscopy and the successful immobilization of the peptide on the silica support was confirmed. This grafted phase was packed into a stainless steel column and used for mixed-mode chromatography such as reversed-phase high-performance liquid chromatography and hydrophilic interaction liquid chromatography for the efficient separation of hydrophobic compounds, small polar molecules, and drug molecules. Compared with ODS and phenyl columns, this new stationary phase shows considerably higher molecular-planarity selectivity towards polyaromatic hydrocarbons and also available for separation of nucleo-analytes and sulfa-drug molecules in a hydrophilic interaction liquid chromatography mode. The multiple interactions induced by polar carbonyl group and hydrophobic phenyl group allow this peptide-modified silica to serve as a multi-mode stationary phase in high performance liquid chromatography. (C) 2012 Elsevier B.V. All rights reserved.
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