期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1232, 期 -, 页码 176-182出版社
ELSEVIER
DOI: 10.1016/j.chroma.2011.11.056
关键词
Monolithic frit; Capillary electrochromatography; Packed capillaries; Nano-liquid chromatography; Light emitting diode
In this paper the potential of fused silica capillaries packed with RP18 silica particles entrapped with monolithic frits using both nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC) was investigated. Frits were prepared after removing a short part of the polyimide layer on the capillary wall and irradiating the polymerization mixture with an UV-light emitter diode (LED) at 370 nm. The capillary, was rotated during the polymerization procedure in order to obtain a homogeneous monolith. The distance of the LED from the capillary and the exposure time to UV light were studied in order to obtain frits with good porosity and high robustness. A mixture containing five alkylbenzenes was selected as sample and analyzed by both nano-LC and CEC. The standard mixture was baseline separated with good efficiency in the range 78,000-93.000 and 99,000-113,000 plates/m in nano-LC and CEC, respectively. The columns resulted to be very robust and the prepared monolithic frits allowed working with backpressure as high as 400 bar (nano-LC). In addition high voltages were applied in CEC (25-30 kV) without bubbles formation in absence of pressure assistance during runs. (C) 2011 Elsevier B.V. All rights reserved.
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