期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 12, 页码 1576-1584出版社
ELSEVIER
DOI: 10.1016/j.chroma.2011.01.055
关键词
Dispersive liquid-liquid microextraction; Gas chromatography-tandem mass spectrometry; Cork taint; Brett character; Wine; Experimental design
资金
- Autonomous Government of La Rioja
- Consejeria de Educacion
- Cultura y Deporte
- Spanish Government, Ministerio de Ciencia e Innovacion [CTQ2008-03493/BQU]
A novel dispersive liquid-liquid microextraction (DLLME) method, coupled to gas chromatography-tandem mass spectrometry (GC-MS/MS), was developed for simultaneously determining the main compounds responsible for cork taint (2,4,6-trichloranisole (TCA), 2,3,4,6-tetrachloroanisole (TeCA), 2,4.6-tribromoanisole (TBA) and pentachloranisole (PCA)) and Brett character (4-ethylguaiacol (EG), 4-ethylphenol (EP), 4-vinylguaiacol (VG) and 4-vinylphenol (VP)) in wines. Optimisation of DLLME procedure was performed by evaluating the type of disperser and extraction solvents and the temperature and salt addition effects. The volumes of disperser and extraction solvents were also optimised by means of a central composite design combined with desirability functions. Under optimum conditions, 5 mL of wine were extracted with an extraction mixture consisting of 1.43 mL of acetone, and 173 mu L of chloroform at room temperature. The analytical characteristics of the method were evaluated. Satisfactory linearity (with correlation coefficients over 0.992), repeatability (below 11.6%) and between-days precision (below 11.0%) were obtained for all target analytes. Detection limits attained were at similar levels or even lower than the olfactory threshold of the studied compounds. Finally, the developed method was successfully applied to the analysis of wine samples. To our knowledge, this is the first time that DLLME has been applied to simultaneously determine the compounds responsible for cork taint and Brett character in wine. (C) 2011 Elsevier B.V. All rights reserved.
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