期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 29, 页码 4593-4598出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.05.037
关键词
Oliveria decumbens Vent.; Ultrasonic assisted extraction; Dispersive liquid-liquid microextraction; Experimental design; Gas chromatography; Essential oil
Ultrasonic assisted extraction-dispersive liquid-liquid microextraction (UAE-DLLME) coupled with gas chromatography (GC) was applied for extraction and determination of essential oil constituents of the plant Oliveria decumbens Vent. Scanning electron microscopy (SEM) was used to see the effect of ultrasonic radiation on the extraction efficiency. By comparison with hydrodistillation. UAE-DLLME is fast, low cost, simple, efficient and consuming small amount of plant materials (similar to 1.0 g). The effects of various parameters such as temperature, ultrasonication time, volume of disperser and extraction solvents were investigated by a full factorial design to identify significant variables and their interactions. The results demonstrated that temperature and ultrasonication time had no considerable effect on the results. In the next step, a central composite design (CCD) was performed to obtain the optimum levels of significant parameters. The obtained optimal conditions were: 0.45 mL for disperser solvent (acetonitrile) and 94.84 mu L for extraction solvent (chlorobenzene). The limits of detection (LODs), linear dynamic range and determination coefficients (R-2) were 0.2-29 ng mL(-1), 1-2100 ng mL(-1) and 0.995-0.998, respectively. The main components of the essential oil were: thymol (47.06%), carvacrol (23.31%), gamma-terpinene (18.94%), p-cymene (8.71%), limonene (0.76%) and myristicin (0.63%). (C) 2011 Elsevier B.V. All rights reserved.
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