期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 3, 页码 504-517出版社
ELSEVIER
DOI: 10.1016/j.chroma.2010.11.008
关键词
Comprehensive two-dimensional gas chromatography; GC x GC; HS-SPME; Wine; Volatile profiling; Aroma; Cabernet Sauvignon
资金
- Australian Federal Government
- Houghton Wines
- Australian Research Council (ARC)
Future understanding of differences in the composition and sensory attributes of wines require improved analytical methods which allow the monitoring of a large number of volatiles including those present at low concentrations. This study presents the optimization and application of a headspace solid-phase microextraction (HS-SPME) method for analysis of wine volatiles by comprehensive two-dimensional gas chromatography (GC x GC) time-of-flight mass spectrometry (TOFMS). This study demonstrates an important advancement in wine volatile analysis as the method allows for the simultaneous analysis of a significantly larger number of compounds found in the wine headspace compared to other current single dimensional GC-MS methodologies. The methodology allowed for the simultaneous analysis of over 350 different tentatively identified volatile and semi-volatile compounds found in the wine headspace. These included potent aroma compound classes such as monoterpenes, norisoprenoids, sesquiterpenes, and alkyl-methoxypyrazines which have been documented to contribute to wine aroma. It is intended that wine aroma research and wine sensory research will utilize this non-targeted method to assess compositional differences in the wine volatile profile. (C) 2010 Published by Elsevier B.V.
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