期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 37, 页码 6540-6547出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.06.108
关键词
Pesticide residue analysis; Clean-up; Matrix effects; High-throughput planar solid phase extraction; HTpSPE; TLC-MS interface; Liquid chromatography-mass spectrometry (LC-MS)
Efficient clean-up is indispensable for preventing matrix effects in multi-residue analysis of pesticides in food by liquid and gas chromatography coupled to mass spectrometry. As a completely new approach, highly automated planar chromatographic tools were applied for powerful clean-up, called high-throughput planar solid phase extraction (HTpSPE). Thin-layer chromatography (TLC) was used to completely separate pesticides from matrix compounds and to focus them into a sharp zone, followed by extraction of the target zone by the TLC-MS interface. HTpSPE resulted in extracts nearly free of interference and free of matrix effects, as shown for seven chemically representative pesticides in four different matrices (apples, cucumbers, red grapes, tomatoes). Regarding the clean-up step, quantification by LC-MS provided mean recovery (against solvent standards) of 90-104% with relative standard deviations of 0.3-4.1% (n = 5) for two spiking levels of 0.1 and 0.5 mg/kg. Clean-up of one sample was completed in a manner of minutes, while running numerous samples in parallel at reduced costs, with very low sample and solvent volumes. (C) 2011 Elsevier B.V. All rights reserved.
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