期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 45, 页码 8150-8155出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.09.019
关键词
Molecularly imprinted polymer; In situ synthesis; HPLC; Semi-preparative; Emodin
资金
- National Key Special Project of Science and Technology for Innovation Drugs [2008ZX09401-006, 2009ZX09502-015]
- National science and technology [2008BAI51BOO]
In this work, we prepared a monolithic and surface initiated molecularly imprinted polymeric (MIP) column for HPLC and explored its application for template separation from plant extract. The silica beads (40-60 mu m) were coupled with initiator on the surface and then packed in to a stainless steel HPLC column. The pre-polymerization mixture (the template, functional monomer and crosslinker were emodin, acrylamide and divinylbenzene, respectively) was injected into the column and polymerized by thermal initiation. The prepared MIP column exhibited excellent retention capability and large imprinted factor for template (the retention time and imprinted factor for emodin on MIP column were 16.26 min and 7.21, respectively). Moreover, the emodin-molecularly imprinted polymeric column could be applied to separate emodin from alcohol extract of Rheum palmatum L. at semi-preparative scale and 1.2 mg of emodin was obtained in 4h. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
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