期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 13, 页码 1749-1755出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.01.079
关键词
Orthogonal parallel separation; Retention time; Accurate molecular weight; Chemical identification; Flavonoids; Library searching
资金
- National Key Technology R&D Program in the 11th 5-year Plan of China [2009BADB9B02]
- Major National Sci-Tech Projects [2009ZX09301-012, 2009ZX09313-003]
- National Science Foundation of China [30801513]
Recent advances in the theory and application of orthogonal LC separation have allowed for the establishment of a more effective method for the chemical identification of target compounds in complex samples, especially structurally similar compounds. In this study, a new chemical identification method based on orthogonal parallel separation and accurate molecular weight confirmation was developed. An orthogonal separation system consisting of an XTerra MS C-18 column, a home-made Click OEG column, and a Click CD column was established for separation and identification. In addition, 82 flavonoids were selected as references, to be used for the construction of a library. Retention times of each reference flavonoid on each column and accurate molecular weights were recorded and imported into a searchable library as tags for the unknown screening. For the method validation, two complex mixtures, fractions of Dalbergia odorifera T. Chen and Scutellaria baicalensis Georgi, specifically, were separated and identified. In total, nine compounds were unequivocally identified by retention time and confirmation of accurate molecular weight, demonstrating that this method is suitable and efficient for the chemical identification of complex samples. (C) 2011 Elsevier B.V. All rights reserved.
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