期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 20, 页码 2865-2870出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.02.064
关键词
Enantiomers; Lansoprazole; Matrix effects; Native samples; Pantoprazole; Tandem mass-spectrometry
资金
- Fundacao de Amparo a Pesquisa e Desenvolvimento do Estado de Sao Paulo (FAPESP) [2006/05126-9, 2008/05778-1]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [471775/2008-8]
A two-dimensional liquid chromatography system coupled to ion-trap tandem mass spectrometer (2DLC-IT-MS/MS) was employed for the simultaneous quantification of pantoprazole and lansoprazole enantiomers fractions. A restricted access media of bovine serum albumin octyl column (RAM-BSA C-8) was used in the first dimension for the exclusion of the humic substances, while a polysaccharide-based chiral column was used in the second dimension for the enantioseparation of both pharmaceuticals. The results described here show good selectivity, extraction efficiency, accuracy, and precision with detection limits of 0.200 and 0.150 mu g L-1 for the enatiomers of pantoprazole and lansoprazole respectively, while employing a small amount (1.0 mL) of native water sample per injection. This work reports an innovative assay for monitoring work, studies of biotic and abiotic enantioselective degradation and temporal changes of enantiomeric fractions. (C) 2011 Elsevier B.V. All rights reserved.
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