期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 52, 页码 9428-9434出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.11.013
关键词
Carbon nanotubes; Ionic liquids; Ionic liquids-coated multi-walled carbon nanotubes; Micellar electrokinetic chromatography; Multi-walled carbon nanotubes; Qishenyiqi dropping pills
资金
- China Postdoctoral Science Special Foundation [201104740]
- National Science Foundation of China [81130068]
A new CE system using ionic liquids coated multi-walled carbon nanotubes (ILs-MWNTs) as pseudostationary phase was developed for the simultaneous determination of four flavonoids, four phenolic acids and two saponins. Several parameters affecting the separation were studied, including the choice of ILs, ILs-MWNTs concentration, the respective use of ILs and MWNTs, buffer pH, SDS concentration and borate content. Results revealed that the addition of ILs-MWNTs in running electrolytes enhanced the separation of target compounds compared to conventional micelle because the surface of carbon nanotubes interacted favorably with the analytes. Under the optimum conditions, a baseline separation was achieved for these analytes within 11 min in a 41.5 cm of effective length fused-silica capillary. At a voltage of 28.0 kV, the separation was carried out in a 10 mM borate buffer (pH 9.0) containing 100 mM SDS, 6% propanol and 4 mu g mL(-1) ILs-MWNTs. All calibration curves showed good linearity (r(2) >0.9990) within the test ranges. The intra- and inter-day precisions as determined from standard solutions were below 3.30% and 6.23%, respectively. The recoveries for ten compounds were found to range from 85.5 to 101.8%. The method was successfully applied for the determination of three types of compounds in Qishenyiqi dropping pills. Our experimental results indicated that the proposed method offered new opportunities for the analysis of complex samples. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据