期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1218, 期 20, 页码 2995-3001出版社
ELSEVIER
DOI: 10.1016/j.chroma.2011.03.037
关键词
Void volume; Accessible volume; Tracer pulse chromatography
资金
- National Science Foundation [CHE-0715094]
- University of Mississippi
The experimental technique of mass spectrometric tracer pulse chromatography was used to determine the void volume, i.e., the total volume of eluent in the column, and the volume of eluent moving freely through the column, i.e., mobile phase volume, for a series of eluents with a C-18-bonded RPLC column. The interstitial volume of the column was determined by size exclusion chromatography. In order to evaluate the utility of the accessible volumes determined from the retention volumes of homologous solutes, the accessible volume of the column was determined as a function of eluent composition and temperature with polystyrene and polyethylene glycol samples using Martin's Rule. Comparison of these four measured volumes indicated that the experimentally measured accessible volumes did not correspond to either the void volumes, mobile phase volumes or interstitial volumes. (C) 2011 Elsevier B.V. All rights reserved.
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