期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1217, 期 8, 页码 1414-1418出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.12.031
关键词
Metabolomics; Herbal extract; Quality control; HPLC-UV
资金
- Ph.D. Programs Foundation of Ministry of Education of China [200806101089]
A lack of adequate or accepted research methodology has been a major obstacle to study herbal medicines In this study, instead of the prevalent hyphenated chromatographies, common high performance liquid chromatography equipped with ultraviolet detector (HPLC-UV) and multivariate statistical analysis were utilized to assess the qualities of total flavones of sea buckthorn (TFS), an 85% ethanol extract of the sea buckthorn berries. Two complementary HPLC-UV methods were developed, validated and combined to comprehensively determine the ingredients in TFS Principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) of the combined analytical data showed that the six batches of TFS could be well differentiated Hierarchical cluster analysis (HCA) using Ward's minimum variance method of the PLS-DA loading matrix demonstrated the known ingredients (quercetin, kaempferol, isorhamnetin, oleanolic acid and ursolic acid) and three unknown ingredients in TFS significantly contributed to the quality differences A PLS regression model indicated that the results of the present method correlated well with the content of total flavones, which is now the quality control approach of ITS Results from this study indicated that the proposed method is reliable for the duality reassessment of some widely used herbal extracts Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved
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