Evaluation of the enantioselectivity of glycogen-based dual chiral selector systems towards basic drugs in capillary electrophoresis

Several chiral reagents including cyclodextrins (CDs) and derivatives crown ethers proteins chiral surfactants and polymers have been involved in dual selector systems for enantioseparation of a series of chiral compounds by capillary electrophoresis (CE) In comparison to the chiral reagents above-mentioned there is no report concerning the use of polysaccharides in dual chiral CE system In this paper we first investigate the enantioselectivity of polysaccharide-based dual selector systems towards some chiral drugs During our recent work glycogen belonging to the class of branched polysaccharides has been used as a novel chiral selector in CE In this study three glycogen-based dual chiral CE systems have been established for enantiomeric separations of several racemic basic drugs consisting of duloxetine cetirizine citalopram sulconazole laudanosine amlodipine propranolol atenolol and nefopam These three dual systems combined glycogen (neutral polysaccharide) with chondroitin sulfate A (CSA ionic polysaccharide) beta-CD and HP-beta-CD respectively It was found that the dual system of glycogen/CSA exhibited good enantioselective properties toward the tested drugs More importantly compared to the single selector systems synergistic effect was observed when glycogen was used with CSA for most of the analytes This indicated the enhancement of enantioseparation observed for these analytes in glycogen/CSA system might be due to some favorable interaction effects between glycogen and CSA Moreover in order to evaluate the stereoselectivity of glycogen/CSA the influences of buffer pH and selector concentration on enantioseparation of the studied drugs were also investigated (C) 2010 Elsevier B V All rights reserved