期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1217, 期 48, 页码 7605-7611出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.10.006
关键词
Capillary electrophoresis; Electrospray ionization; Intact proteins; Mass spectrometry; Sheathless interfacing; Sheath liquid interfacing
资金
- Dutch Technology Foundation STW Applied Science Division of NWO
- Ministry of Economic Affairs
The performance of a prototype porous tip sprayer for sheathless capillary electrophoresis-mass spectrometry (CE-MS) of intact proteins was studied Capillaries with a porous tip were inserted in a stainless steel needle filled with static conductive liquid and installed in a conventional electrospray ionization (ESI) source Using a BCE of 100 mM acetic acid (pH 3 1) and a positively charged capillary coating a highly reproducible and efficient separation of four model proteins (insulin carbonic anhydrase II ribonuclease A and lysozyme) was obtained The protein mass spectra were of good quality allowing reliable mass determination of the proteins and some of their impurities Sheath-liquid CE-MS using the same porous tip capillary and an isopropanol-water-acetic acid sheath liquid showed slightly lower to similar analyte responses However as noise levels increased with sheath-liquid CE-MS detection limits were improved by a factor 6 5-20 with sheathless CE-MS The analyte response in sheathless CE-MS could be enhanced using a nanoESI source and adding 5% isopropanol to the BCE leading to improved detection limits by 50-fold to 140-fold as compared to sheath liquid interfacing using the same capillary - equivalent to sub-nM detection limits for three out of four proteins Clearly the sheathless porous tip sprayer provides high sensitivity CE-MS of intact proteins (C) 2010 Elsevier B V All rights reserved
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