Quantitative determination of triclocarban in wastewater effluent by stir bar sorptive extraction and liquid desorption-liquid chromatography-tandem mass spectrometry

Triclocarban is all antimicrobial and antibacterial agent found in personal care products and subsequently is a prevalent wastewater contaminant A quantitative method was developed for the analysis of triclocarban in wastewater effluents using Stir bar sorptive extraction-liquid desorption (SBSE-LD) followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) by means of an electrospray interface A Stir bar coated with polydimethylsiloxane (PDMS) is placed within a vial containing wastewater effluent and is stirred for an hour at room temperature The PDMS stir bar is then placed in a LC vial containing methanol and is desorbed in a sonicator bath The methanol is evaporated to dryness and reconstituted in 75% methanol Spike and recovery experiments in groundwater that did not contain native concentrations of triclocarban were performed at 0 5 mu g/L and were 93 +/- 8% Recoveries in wastewater effluent that were corrected for the background levels of triclocarban were 92 +/- 2% and 96 +/- 5%, respectively, when spiked with 0 5 and 5 mu g/L of triclocarban. The precision of the method as indicated by the relative standard error was 2% The limit of quantitation was 10 ng/L. The SBSE-LD-LC/MS/MS method was applied to wastewater effluent samples collected from northeast Ohio Triclocarban was quantitated in all five effluent samples, and its concentration ranged from 50 to 330ng/L., The described method demonstrates a simple. green, low-sample volume, yet, sensitive method to measure triclocarban in aqueous matrices (C) 2010 Elsevier B V All rights reserved