期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1217, 期 47, 页码 7455-7459出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.09.064
关键词
Tetrodotoxin; C(9)-base; GC-MS; Puffer; LLE
资金
- Universiti Sains Malaysia [304/CRACUN/639057]
Tetrodotoxin (TTX), a toxic compound found in some puffers can cause death to humans through consumption. We have developed a simplified method for the screening of TTX in puffers using GC-MS. A puffer tissue of 0.5 g was treated with 5 mL of 0.1% acetic acid, followed by alkaline hydrolysis, LLE or liquid-liquid extraction and N-methyl-N-TMS-trifluoroacetamide derivatization. The developed method used only a small sample and solvent, simplified LLE and derivatization procedures and short chromatographic analysis (8.2 min). All of these contribute to cost-saving, enhanced sample throughput and high sensitivity of the screening assay. The developed method was validated and proved to be within the acceptable range. (C) 2010 Elsevier B.V. All rights reserved.
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