Tree-shaped paper strip for semiquantitative colorimetric detection of protein with self-calibration

This paper described a convenient semiquantitative method for calorimetric detection of protein with self-calibration integrated on the test strip. Hydrophilic paper was employed as microfluidic device for running calorimetric assay, tree-shaped design was developed to ensure uniform microfluidic flow for multiple branches. The approach was validated with bovine serum albumin (BSA) calorimetric detection, and colorimetric results observed by naked eyes were consistent with that from apparatus. The device could be coupled with digital transmission of images for remote monitoring system for diagnosis, food control, and environmental analysis. (C) 2010 Elsevier B.V. All rights reserved.