期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1217, 期 45, 页码 7144-7152出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.09.028
关键词
Rhubarb decoction; Metabolites; In vivo metabolism; HPLC-DAD; HPLC-MS/MS
资金
- State Administration of Traditional Chinese Medicine [06-07ZP17]
- Chinese Natural Science Fund [30672587]
- Technology Innovation Program for post-graduate of Jiangsu province [CX08B_194Z)]
High-performance liquid chromatography with diode-array detection (HPLC-DAD) and tandem mass spectrometry (HPLC-MS/MS) was used for separation and identification of metabolites in rat urine bile and plasma after oral administration of rhubarb decoction Based on the proposed strategy 91 of the 113 potential metabolites were tentatively identified or characterized Besides anthraquinones metabolites gallic acid (-)-epicatechin and (+)-catechin metabolites were also detected and characterized in these biological samples Our results indicated that glucuronidation and sulfation were the main metabolic pathways of anthraquinones while methylation glucuronidation and sulfation were the main metabolic pathways of gallic acid (-)-epicatechin and (+)-catechin Phase I reactions (e g hydroxylation and reduction) played a relatively minor role compared to phase II reactions in metabolism of phenolic compounds of rhubarb decoction The identification and structure elucidation of these metabolites provided essential data for further pharmacological and clinical studies of rhubarb and related preparations Moreover the results of the present Investigations clearly indicated the relevance and usefulness of the combination of chromatographic spectrophotometric and mass-spectrometric analysis to detect and identify metabolites (C) 2010 Elsevier BV All rights reserved
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