Comparison of two different isolation methods of benzimidazoles and their metabolites in the bovine liver by solid-phase extraction and liquid chromatography-diode array detection

A new analytical method that uses high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the analysis of 14 benzimidazoles residues, including metabolites, in bovine liver Samples were extracted using two different extraction procedures with phosphate buffer after enzymatic hydrolysis (method A) or using organic solvent, i e acetonitrile (method B) Then, samples were purified on a strong cation exchange (SCX) cartridge and analyzed in HPLC/DAD The recovery percentages,obtained spiking the matrix (liver) at concentrations of 500 and 100 mu g kg(-1) with a standard Mixture of benzimidazoles, were in the range 6-101% and 80-102% for methods A and B. respectively The repeatability of the methods was assessed in all cases by the % of correlation Value (CV) that was lower than 19%. The limits Of quantification (LOQs) in the matrix for methods A and 13 were in the range 40-60 and 20-50 mu g kg(-1), respectively The best of the two methods. method B. was used for the analysis of 10 bovine liver samples (C) 2010 Elsevier B V All rights reserved