期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1217, 期 30, 页码 4958-4964出版社
ELSEVIER
DOI: 10.1016/j.chroma.2010.05.051
关键词
Chitosan functionalized monolithic silica column; Hydrophilic interaction chromatography; Acidic compounds
资金
- National Natural Science Foundation of China [20575041]
- Ministry of Education Science Foundation of China for Returnees
- Special Foundation of Guangzhou for Public Instruments
A novel cationic hydrophilic interaction monolithic stationary phase based on the chemical modification of carboxymethyl chitosan (CMCH) to the monolithic silica skeleton using carbodiimide as an activation reagent was prepared for performing capillary liquid chromatography. The amino and hydroxy moieties of CMCH functioned as both the ion-exchange sites and polar providers. The performance of the column was studied by the separation of polar acidic compounds. The chitosan functionalized monolithic silica column showed good selectivity for nucleosides, nucleotides, aromatic acids and aliphatic acids. The mechanism for the separation of these compounds was also studied. The results showed that these compounds were separated primarily based on the hydrophilic interaction mechanism. (C) 2010 Elsevier B.V. All rights reserved.
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