期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1217, 期 16, 页码 2581-2588出版社
ELSEVIER
DOI: 10.1016/j.chroma.2009.10.072
关键词
Packed 96-tips; Automated sample preparation; Methacrylate monolith; LC-MS/MS; Plasma and blood samples
In the pharmaceutical industry the growing number of samples to be analyzed requires high throughput and fully automated analytical techniques. Commonly used sample-preparation methods are solid-phase extraction (SPE), liquid-liquid extraction (LLE) and protein precipitation. In this paper we will discus a new sample-preparation technique based on SPE for high throughput drug extraction developed and used by our group. This new sample-preparation method is based on monolithic methacrylate polymer as packing sorbent for 96-tip robotic device. Using this device a 96-well plate could be handled in 2-4 min. The key aspect of the monolithic phase is that monolithic material can offer both good binding capacity and low back-pressure properties compared to e.g. silica phases. The present paper presents the successful application of monolithic 96-tips and LC-MS/MS by the sample preparation of busulphan, rescovitine, metoprolol, pindolol and local anaesthetics from human plasma samples and cyklophosphamid from mice blood samples. (C) 2009 Elsevier B.V. All rights reserved.
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