期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 15, 页码 3163-3167出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.01.096
关键词
Nitrite; Nitrate; Peroxynitrite; Photochemical reaction; Luminol; Chemiluminescence
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [19750066]
- Grants-in-Aid for Scientific Research [19750066, 22404002] Funding Source: KAKEN
A simple. sensitive and selective method for the simultaneous determination of nitrite and nitrate in water samples has been developed. The method is based on ion-exchange separation, online photochemical reaction, and luminol chemiluminescence detection. The separation of nitrite and nitrate was achieved using an anion-exchange Column with a 20 mM borate buffer (pH 10.0). After the separation, these ions were converted to peroxynitrite by online UV irradiation using a low-pressure mercury lamp and then mixed with a luminol solution prepared with carbonate buffer (pH 10.0). The calibration graphs of the nitrite and nitrate were linear in the range from 2.0 x 10(-9) to 2.5 x 10(-6) M and 2.0 x 10(-8) to 2.5 x 10(-5) M, respectively. Since the sensitivity of nitrite was about 10 times higher than that of nitrate, the simultaneous determination of nitrite and nitrate in the water samples could be efficiently achieved. This method was Successfully applied to various water samples - river water. pond water, rain water, commercial mineral water, and tap water - with only filtration and dilution steps. (C) 2009 Elsevier B.V. All rights reserved.
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