期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 44, 页码 7558-7563出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.04.040
关键词
Boronate affinity; Capillary electrophoresis; cis-Diol; Extraction; Magnetic nanoparticles
In recent years. functionalized magnetic nanoparticles (MNPs) have drawn continuously increasing attention due to their great potential for capturing biological molecules or species However, functionalized MNPs as nanoextraction probes and the coupling with a separation platform for chemical analysis have not extensively investigated yet. in this study, boronare functionalized MNPs were synthesized and employed as extracting probes to Capture and enrich cis-diol-containing biomolecules, and an off-line coupling method of the MNPs-based extraction with capillary electrophoresis (CE) was established by using pH junction, an on-line preconcentration technique in CE, as a bridge for the coupling. The prepared MNPs exhibited specific selectivity and sufficient capacity The pH junction compressed a large injected sample volume into a much narrower sample zone and therefore significantly improved the detection sensitivity, solving the sensitivity mismatch between the MNPs-based extraction and CE. Experimental conditions for the pH junction and the desorption were optimized. Under the optimized conditions, the sensitivity was enhanced by 42-fold as compared with regular CE. N,N-dimethylformamide was found to be an effective desorption promoter, which reduced the desorption time to a few minutes. With the established method, riboflavin in a human urine sample was determined. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据