期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 28, 页码 5391-5397出版社
ELSEVIER
DOI: 10.1016/j.chroma.2009.05.038
关键词
Lemon verbena; Phenolic compounds; High-performance liquid chromatography; Mass spectrometry
资金
- Spanish Ministry of Education and Science [AGL2007-62806, AGL2008-05108-C03-03]
- FPU [AP2007-03246]
- Andalusian Regional Government Council of Innovation and Science [P07-AGR-02619]
High-performance liquid chromatography with diode array and electrospray ionization mass spectrometric detection was used to carry out the comprehensive characterization of a lemon verbena extract with demonstrated antioxidant and antiinflammatory activity. Two different MS techniques have been coupled to HPLC: on one hand, time-of-flight mass spectrometry, and on the other hand. tandem mass spectrometry on an ion-trap. The use of a small particle size C18 column (1.8 mu m) provided a great resolution and made possible the separation of several isomers. The UV-visible spectrophotometry was used to delimit the class of phenolic compound and the accurate mass measurements on time-of-flight spectrometer enabled to identify the compounds present in the extract. Finally, the fragmentation pattern obtained in MS-MS experiments confirmed the proposed structures. This procedure was able to determine many well-known phenolic compounds present in lemon verbena such as verbascoside and its derivatives, diglucuronide derivatives of apigenin and luteolin, and eukovoside. Also gardoside, verbasoside, cistanoside F, theveside, campneoside I, chrysoeriol-7-diglucuronide, forsythoside A and acacetin-7-diglucuronide were found for the first time in lemon verbena. (C) 2009 Elsevier B.V. All rights reserved.
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