期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 37, 页码 6466-6470出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.07.047
关键词
Commercial instrument; In-line coupling; Large volume stacking using an electroosmotic flow pump; Nonaqueous capillary electrophoresis; Single drop microextraction
资金
- Seoul RBD program
- Ministry of Education, Science and Technology in Korea
In order to improve the concentration sensitivity of capillary electrophoresis (CE), two sample preconcentration techniques, single drop microextraction (SDME) and large volume stacking using an electroosmotic flow pump (LVSEP), were coupled in-line in a commercial CE instrument. By simple programming of liquid handling sequences, a pentanol drop was prepared at the tip of a fused silica capillary over which a Teflon tube had been sleeved to serve as a hydrophobic support. After extraction of the analytes from an aqueous donor solution into the drop, the entire capillary column was filled with enriched pentanol extract. LVSEP, in which the sample matrix is automatically removed by the EOF, was then carried out using a methanolic run buffer. The overall enrichment factors for the analytes pentachlorophenol (PCP), 3-bromobenzoic acid (3-BBA), and 4-iodobenzoic acid (4-IBA), from a combination of 30 min SDME and LVSEP on a 27 cm capillary, were about 7000, even without agitation of the donor solution. The resulting limits of detection for PCP, 3-BBA, and 4-IBA were 0.7, 0.3 and 0.7 nM, respectively. Since no modification of the existing CE instrument is necessary and a bare capillary is used for LVSEP, this scheme can be adapted quite easily for many CE applications that require high concentration sensitivity. (C) 2009 Elsevier B.V. All rights reserved.
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