期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1216, 期 21, 页码 4560-4567出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.03.056
关键词
Surface molecular imprinting; Silica beads; beta-Cyclodextrin; Lysozyme; High-performance liquid chromatography; Protein separation
资金
- National Natural Science Foundation of China [20875049]
- National Basic Research Program of China (973 Program) [2007CB914100, 2006CB705703]
A novel protein imprinted polymer for selective recognition of lysozyme was obtained. Acryloy-beta-cyclodextrin, which offered hydrophilic exterior and hydrophobic cavity that were allowed to self-assemble with the template protein through hydrogen interaction and hydrophobic interaction, was synthesized and used as the functional monomer. Polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a new type of protein imprinted polymer. Langmuir adsorption model was employed to describe the isotherms. and maximum adsorption capacity was evaluated. The performance of such imprinted polymer was further demonstrated by high-performance liquid chromatography, and the results showed that the column packed with the lysozyme imprinted silica beads could effectively separate lysozyme from the mixture of lysozyme-cytochrome c, lysozyme-bovine serum albumin, lysozyme-avidin or lysozyme-methylated bovine serum albumin, which showed its high selectivity. (C) 2009 Elsevier B.V. All rights reserved.
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