期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1185, 期 1, 页码 145-150出版社
ELSEVIER
DOI: 10.1016/j.chroma.2008.01.032
关键词
DNA methylation; methylcytosine; short-end injection; capilllary electrophoresis
We propose a new capillary zone electrophoresis method applying short-end injection technique for the fast evaluation of methylcystosine/total cytosine ratio after acidic DNA hydrolysis. By short-end injection and by using a 100 mmol/l Tris solution titrated with 1 mol/l phosphoric acid to pH 3.75 as background electrolyte, cytosine and methylcytosine were separated with a good resolution in less than 1.5 min. Stepwise multiple linear regression with DNA methylation degree as the dependent variable and age, cysteine, homocysteine and methionine as independent variables, showed a negative association with age and that total cysteine is the most important determinant of DNA methylation. (C) 2008 Elsevier B.V. All rights reserved.
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