期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1204, 期 1, 页码 28-34出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.07.010
关键词
HPLC; column-switching; HILIC; hydrophobic; hydrophilic; metabonomics
资金
- China International Science and Technology Cooperation Program [2007DFA31060]
- National Key Project of Scientific and Technical Supporting Programs [2006BAK02A12]
- National Basic Research Program of China [2007CB914701]
- Sino-German Center for Research Promotion [364]
- National Natural Science Foundation of China [20675082, 20425516]
- Chinese Academy of Sciences [K2006A12, K2006A14]
in this study we developed and optimized a column-switching high-performance liquid chromatographic (HPLC) system for the complementary analysis of polar and apolar compounds in complex samples. The polar compounds are separated on a hydrophilic interaction chromatographic (HILIC) column; the part of the sample non-retained on HILIC is transferred into and separated on a reversed-phase (RP) column through an interface, thus preventing the loss of analytes eluting at dead time in common single-column mode. The signals are in turn recorded in the same chromatogram either by a UV or by a mass spectrometric detector. Applying a mixture of 25 standard compounds (12 hydrophilic and 13 hydrophobic) the system proves to be reliable, robust and easy-operating. The investigation of urine revealed an increase in the detected metabolite ion masses compared to the single-column HILIC-electrospray ionization (ESI)-MS analysis. Utilizing online the complementary selectivity of HILIC and RPLC in a fully automatic mode, this approach holds out the prospect to enlarge the number of detectable compounds in non-targeted -omics studies and sophisticated target-driven approaches by a single injection. (c) 2008 Elsevier B.V. All rights reserved.
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