期刊
ACTA BIOMATERIALIA
卷 24, 期 -, 页码 87-95出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.05.035
关键词
Polymer nanosheet; Poly(lactic acid); Silver sulfadiazine; Antimicrobial; Wound dressing
资金
- JSPS [25289252]
- National Defense Medical College
- Grants-in-Aid for Scientific Research [15H05355, 25293369] Funding Source: KAKEN
Partial-thickness burn injury has the potential for reepithelialization and heals within 3 weeks. If the wound is infected by bacteria before reepithelization, however, the depth of disruption increases and the lesion easily progresses to the full-thickness dermal layers. In the treatment of partial-thickness burn injury, it is important to prevent the wound area from bacterial infection with an antimicrobial dressing. Here, we have tested the antimicrobial properties of polymeric ultra-thin films composed of poly(lactic acid) (termed PLA nanosheets), which have high flexibility, adhesive strength and transparency, and silver sulfadiazine (AgSD), which exhibits antimicrobial efficacy. The AgSD-loaded nanosheet released Ag+ for more than 3 days, and exerted antimicrobial efficacy against methicillin-resistant Staphylococcus aureus (MRSA) in an in vitro Kirby-Bauer test. By contrast, a cell viability assay indicated that the dose of AgSD used in the PLA nanosheets did not show significant cytotoxicity toward fibroblasts. In vivo evaluation using a mouse model of infection in a partial-thickness burn wound demonstrated that the nanosheet significantly reduced the number of MRSA bacteria on the lesion (more than 10(5)-fold) and suppressed the inflammatory reaction, thereby preventing a protracted wound healing process. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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