期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1189, 期 1-2, 页码 332-338出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2007.11.082
关键词
proteomics; human serum; human plasma; protein depletion; immunoaffinity depletion; protein fractionation
The tremendous complexity of the serum and plasma proteome presents extreme analytical challenges in comprehensive analysis due to the wide dynamic range of protein concentrations. Therefore, robust sample preparation methods remain one of the important steps in the proteome characterization workflow. We present the results on a new column for the specific depletion of 14 high-abundant proteins from human serum and plasma and the subsequent reversed-phase fractionation of the flow-through proteins. Analysis of tryptic peptides was accomplished with microfluidic HPLC-Chip/MS system. Results indicate that high-abundant protein depletion combined with RP fractionation of plasma showed an improved dynamic range for proteomic analysis and enabled the identification of low-abundant plasma proteins. (c) 2007 Elsevier B.V. All rights reserved.
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