期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1200, 期 1, 页码 55-61出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.03.025
关键词
microfluidics; HPLC; chip; monoliths; stationary phase; protein separation; peptide separation; proteomics
资金
- NIBIB NIH HHS [EB-006133, R01 EB006133, R01 EB006133-03] Funding Source: Medline
- NIGMS NIH HHS [R01 GM048364-16] Funding Source: Medline
Poly(lauryl methacrylate-co-ethylene dimethacrylate) and poly(styrene-co-divinylbenzene) stationary phases in monolithic format have been prepared by thermally initiated free radical polymerization within polyimide chips featuring channels having a cross-section of 200 mu m x 200 mu m and a length of 6.8 cm. These chips were then used for the separation of a mixture of proteins including ribonuclease A, myoglobin, cytochrome c, and ovalbumin, as well as peptides. The separations were monitored by UV adsorption. Both the monolithic phases based on methacrylate and on styrene chemistries enabled the rapid baseline separation of most of the test mixtures. Best performance was achieved with the styrenic monolith leading to fast baseline separation of all four proteins in less than 2.5 min. The in situ monolith preparation process affords microfluidic devices exhibiting good batch-to-batch and injection-to-injection repeatability. (c) 2008 Elsevier B.V. All rights reserved.
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