期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1212, 期 1-2, 页码 82-88出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.10.036
关键词
Erythropoietin; Recombinant human EPO; Lectin affinity; Wheat germ agglutinin; Lateral flow test
资金
- Phadia AB, Uppsala, Sweden
- Vinnova, Swedish Agency for Innovation system
This work exploits the combination of the lectin affinity chromatography (LAC) with all ultra-sensitive immunochromatographic assay to differentiate several types of erythropoietin (EPC). The chromatographic behaviours of different commercial types of recombinant human EPO (rhEPO), EPO analogues (Aranesp) and urine human EPO (uhEPO) from healthy individuals on eight lectin-Sepharose columns, have been worked out. Results show that when using wheat germ agglutinin (WGA)-Sepharose columns, a careful desorption regime starting with very low concentration (2mM) of the competitive sugar N-acetylglucosamine (GlcNAc) makes it possible to efficiently distinguish endogenous EPO from recombinant EPO and EPO analogues. (C) 2008 Elsevier B.V. All rights reserved.
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