期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1196, 期 -, 页码 28-32出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.05.005
关键词
liquid-phase microextraction; orthogonal array design; fat-soluble vitamins; HPLC
A simple, rapid and efficient microextraction method for the extraction and determination of some fat-soluble vitamins (A, D-2, D-3) in aqueous samples was developed. For the first time orthogonal array designs (OADs) were employed to screen the liquid-phase microextraction (LPME) method in which few microliters of 1-undecanol were delivered to the surface of the aqueous sample and it was agitated for a selected time. Then sample vial was cooled by inserting it into an ice bath for 5 min. The solidified solvent was transferred into a suitable vial and immediately melted. Then, the extract was directly injected into a high-performance liquid chromatography (HPLC) for analysis. Several factors affecting the microextraction efficiency such as sample solution temperature, stirring speed, volume of the organic solvent, ionic strength and extraction time were investigated and screened using an OA(16) (4(5)) matrix. Under the best conditions (temperature, 55 degrees C; stirring speed, 1000 rpm; the volume of extracting solvent, 15.0 mu L; no salt addition and extraction time, 60 min), detection limits of the method were in the range of 1.0-3.5 mu g L-1. The relative standard deviations (RSDs) to determine the vitamins at mu g L-1 levels by applying the proposed method varied in the range of 5.1-10.7%. Dynamic linear ranges of 5-500 mu g L-1 with good correlation coefficients (0.9984 < r(2) < 0.9991) were observed. Finally, the study was applied to determine the vitamins in several real aqueous samples including mixed juice fruit, urine and tap water samples and relatively good results were obtained. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据