期刊
JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷 53, 期 6, 页码 945-952出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/chromsci/bmu155
关键词
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资金
- Construction Scientific Research Projects of National TCM Clinical Research Base of State Administration of TCM China [JDZX2012016]
- Joint Research Projects of Department of Science and Technology of Guangdong Province
- Guangdong Provincial Academy of Chinese Medical Sciences [2011B032200007]
- Provincial Science and Technology project of Department of Science and Technology of Guangdong Province [2013B021800235]
A simple and effective method was established for separation and characterization of flavonoid constituents in Radix Astragali (RA) by combination of ultra-high-pressure liquid chromatography with LTQ-Orbitrap tandem mass spectrometry (u-HPLC-LTQ-Orbitrap-MSn). For three major structural types of flavonoids, the proposed fragmentation pathways and major diagnostic fragment ions of isoflavones, pterocarpans and isoflavans were investigated to trace isoflavonoid derivatives in crude plant extracts. Based on the systematic identification strategy, 48 constituents were rapidly detected and characterized or tentatively identified, many of which were first reported in RA. The u-PHLC-LTQ-Orbitrap MSn platform was proved as an effective tool for rapid qualitative analysis of secondary metabolite productions from natural resources.
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