期刊
NEURON
卷 85, 期 2, 页码 275-288出版社
CELL PRESS
DOI: 10.1016/j.neuron.2014.12.024
关键词
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资金
- NSF Postdoctoral Fellowship in Biology
- Sternlicht Director's Fund Fellowship
- NIH [DP2OD006670, P01 GM099117, R01 MH102416, NS062489, NS073124, NS078164]
- Center for Cell Circuits [P50 HG006193-01]
- New York Stem Cell Foundation
- Harvard Stem Cell Institute
Neuronal development requires a complex choreography of transcriptional decisions to obtain specific cellular identities. Realizing the ultimate goal of identifying genome-wide signatures that define and drive specific neuronal fates has been hampered by enormous complexity in both time and space during development. Here, we have paired high-throughput purification of pyramidal neuron subclasses with deep profiling of spatiotemporal transcriptional dynamics during corticogenesis to resolve lineage choice decisions. We identified numerous features ranging from spatial and temporal usage of alternative mRNA isoforms and promoters to a host of mRNA genes modulated during fate specification. Notably, we uncovered numerous long noncoding RNAs with restricted temporal and cell-type-specific expression. To facilitate future exploration, we provide an interactive online database to enable multidimensional data mining and dissemination. This multifaceted study generates a powerful resource and informs understanding of the transcriptional regulation underlying pyramidal neuron diversity in the neocortex.
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