期刊
NEURON
卷 87, 期 5, 页码 1008-1021出版社
CELL PRESS
DOI: 10.1016/j.neuron.2015.08.005
关键词
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资金
- NRSA fellowship from NINDS [F32 NS070498]
- Timken-Sturgis Foundation
- Japanese Ministry of Education, Culture, Sports, Science, and Technology Long-Term Student Support Program
- National Institute of Neurological Disorders and Stroke [R37NS037116]
- Howard Hughes Medical Institute
- Christopher and Dana Reeve Foundation
- Marshall Foundation
- Sol Goldman Charitable Trust
The coordination of multi-muscle movements originates in the circuitry that regulates the firing patterns of spinal motorneurons. Sensory neurons rely on the musculotopic organization of motorneurons to establish orderly connections, prompting us to examine whether the intraspinal circuitry that coordinates motor activity likewise uses cell position as an internal wiring reference. We generated a motorneuron-specific GCaMP6f mouse line and employed two-photon imaging to monitor the activity of lumbar motorneurons. We show that the central pattern generator neural network coordinately drives rhythmic columnar-specific motorneuron bursts at distinct phases of the locomotor cycle. Using multiple genetic strategies to perturb the subtype identity and orderly position of motorneurons, we found that neurons retained their rhythmic activity-but cell position was decoupled from the normal phasing pattern underlying flexion and extension. These findings suggest a hierarchical basis of motor circuit formation that relies on increasingly stringent matching of neuronal identity and position.
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