期刊
JOURNAL OF CHEMICAL PHYSICS
卷 131, 期 9, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3212597
关键词
biodiffusion; fluorescence; molecular biophysics; proteins
资金
- Swiss National Science Foundation
- Human Frontier Science Program
- National Institutes of Health, NIDDK
Fluorescence intensity correlation functions contain information about photophysical and conformational dynamics. We propose and implement a simple procedure to analyze such functions measured in the presence of resonance energy transfer. When there is a separation of time scales and the conformational dynamics is modeled as diffusion in the potential of mean force along the interdye distance, we obtain an analytic expression for the conformational correlation time. This can be used to find the diffusion coefficient describing conformational fluctuations given the photon count rate and equilibrium distribution.
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