期刊
JOURNAL OF CHEMICAL PHYSICS
卷 131, 期 9, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3216111
关键词
adsorption; atomic force microscopy; biological techniques; DNA; graphite; molecular biophysics
资金
- Swiss National Science Foundation [200021-116515]
- Carlsberg and Novartis Foundations
We have characterized the polymer physics of single-stranded DNA (ssDNA) using atomic force microscopy. The persistence length l(p) of circular ssDNA adsorbed on a modified graphite surface was determined independently of secondary structure. At a very low ionic strength we obtained l(p)=9.1 nm from the bond correlation function. Increasing the salt concentration lead to a decrease in l(p); at 1 mM NaCl we found l(p)=6.7 nm, while at 10 mM NaCl a value l(p)=4.6 nm was obtained. The persistence length was also extracted from the root-mean-square end-to-end distance and the end-to-end distance distribution function. Finally, we have investigated the scaling behavior using the two latter quantities, and found that on long length scales ssDNA behaves as a two-dimensional self-avoiding walk.
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