期刊
JOURNAL OF CHEMICAL ECOLOGY
卷 36, 期 9, 页码 1035-1042出版社
SPRINGER
DOI: 10.1007/s10886-010-9846-7
关键词
Chemical communication; Gas chromatography-mass spectrometry (GC-MS); Human saliva; Stir bar extraction; Volatile organic compounds
资金
- Lilly Chemistry Alumni Chair (Indiana University)
- METACyt Initiative
- Lilly Endowment
- ARO [DAAD19-03-1-0215]
Human saliva not only helps control oral health (with anti-microbial proteins), but it may also play a role in chemical communication. As is the case with other mammalian species, human saliva contains peptides, proteins, and numerous volatile organic compounds (VOCs). A high-throughput analytical method is described for profiling a large number of saliva samples to screen the profiles of VOCs. Saliva samples were collected in a non-stimulated fashion. The method utilized static stir bar extraction followed by gas chromatography-mass spectrometry (GC-MS). The method provided excellent reproducibility for a wide range of salivary compounds, including alcohols, aldehydes, ketones, carboxylic acids, esters, amines, amides, lactones, and hydrocarbons. Furthermore, substantial overlap of salivary VOCs and the previously reported skin VOCs in the same subject group was found in this study by using pattern recognition analyses. Sensitivity, precision, and reproducibility of the method suggest that this technique has potential in physiological, metabolomic, pharmacokinetic, forensic, and toxicological studies of small organic compounds where a large number of human saliva samples are involved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据