期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 34, 期 11, 页码 1749-1760出版社
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2014.137
关键词
Alzheimer's disease; glutamate isotopomers; hypermetabolism; lipoic acid; [1-C-13]glucose metabolism; C-13 nuclear magnetic resonance
资金
- NIH [RO1AG016718, PO1AG026572, K01AG040164]
- LK Whittier Family Foundation
- American Federation for Aging Research grant [A12474]
Alzheimer's disease (AD) is characterized by age-dependent biochemical, metabolic, and physiologic changes. These age-dependent changes ultimately converge to impair cognitive functions. This study was carried out to examine the metabolic changes by probing glucose and tricarboxylic acid cycle metabolism in a 7-month-old triple transgenic mouse model of AD (3xTg-AD). The effect of lipoic acid, an insulin-mimetic agent, was also investigated to examine its ability in modulating age-dependent metabolic changes. Seven-month-old 3xTg-AD mice were given intravenous infusion of [1-C-13]glucose followed by an ex vivo C-13 nuclear magnetic resonance to determine the concentrations of C-13-labeled isotopomers of glutamate, glutamine, aspartate, gamma aminobutyric acid, and N-acetylaspartate. An intravenous infusion of [1-C-13]glucose+[1,2-C-13]acetate was given for different periods of time to distinguish neuronal and astrocytic metabolism. Enrichments of glutamate, glutamine, and aspartate were calculated after quantifying the total (C-12+C-13) concentrations by high-performance liquid chromatography. A hypermetabolic state was clearly evident in 7-month-old 3xTg-AD mice in contrast to the hypometabolic state reported earlier in 13-month-old mice. Hypermetabolism was evidenced by prominent increase of C-13 labeling and enrichment in the 3xTg-AD mice. Lipoic acid feeding to the hypermetabolic 3xTg-AD mice brought the metabolic parameters to the levels of nonTg mice.
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