期刊
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
卷 32, 期 6, 页码 938-951出版社
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2012.42
关键词
cerebral blood volume; forepaw electrical stimulation; hemodynamic response; hemoglobin oxygen saturation; total hemoglobin concentration; transcranial photoacoustic microscopy
资金
- National Science Council, ROC [97-2221-E-007-084-MY3, 96-2220-E-009-029, 97-2220-E-009-029, 99-2221-E-009-154, NSC 99-2911-I-009-101, 100-2911-I-009-101, 100-2321-B-009-001]
- National Tsing Hua University [98N2531E1]
- Ministry of Education, Taiwan
Optical imaging of changes in total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel functional photoacoustic microscopy technique to probe the responses of single cortical vessels to left forepaw electrical stimulation in mice with intact skulls. Functional changes in HbT, CBV, and SO2 in the superior sagittal sinus and different-sized arterioles from the anterior cerebral artery system were bilaterally imaged with unambiguous 36x65-mu m(2) spatial resolution. In addition, an early decrease of SO2 in single blood vessels during activation (i.e., 'the initial dip') was observed. Our results indicate that the initial dip occurred specifically in small arterioles of activated regions but not in large veins. This technique complements other existing imaging approaches for the investigation of the hemodynamic responses in single cerebral blood vessels. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 938-951; doi:10.1038/jcbfm.2012.42; published online 4 April 2012
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据